Journal: iScience
Article Title: Prenatal hormone stress triggers embryonic cardiac hypertrophy outcome by ubiquitin-dependent degradation of mitochondrial mitofusin 2
doi: 10.1016/j.isci.2023.108690
Figure Lengend Snippet: Impairment of mitochondrial dynamics and disruption of energy metabolism occur as a consequence of CORT exposure (A) Morphology of mitochondria of heart of chicken embryos, heart of 4-week-old mice, and H9c2 cells was detected by transmission electron microscopy (TEM). Scale bar: 500 nm. The yellow arrows represent impaired mitochondria. The quantitative analysis of area and aspect ratio of mitochondria was performed by ImageJ software. Center line, median; box, interquartile range; whiskers, range; bars, maximum and minimum. (B) The mitochondria of H9c2 cells were labeled with Mito-Red probe and examined by confocal microscopy. Scale bar: 10 μm. (C and D) The content of ATP in H9c2 cells (n = 3) or heart of chicken embryos (n = 4). In (D), center line, median; box, interquartile range; whiskers, range; bars, maximum and minimum. (E) Seahorse analysis of OXPHOS in H9c2 cells (n = 4). (F) Seahorse analysis of OXPHOS of separated heart mitochondria in 4-week-old mice (n = 5). (G and H) Activities of mitochondrial respiratory chain complex in H9c2 cells (n = 3) or hearts of EDD14 chicken embryo were measured by commercial kits (n = 3–4). Data are expressed as the mean ± SD (unless otherwise indicated) and subjected to statistical analysis using unpaired Student’s t test or one-way ANOVA followed by Dunnett’s multiple comparison test; ns (not significant); ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 vs. the indicated group.
Article Snippet: Mitochondria respiratory chain complex IV assay kit , Nanjing Jiancheng Bioengineering Institute , Cat#: A089-4-1.
Techniques: Disruption, Transmission Assay, Electron Microscopy, Software, Labeling, Confocal Microscopy, Comparison